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pyruvate fermentation to isobutanol (engineered)

State Icon Reaction EC-Number Confidence maximal confidence score from EnzymeDetector
isobutanol + NAD+ <=> H+ + Isobutyraldehyde + NADH 1.1.1.1 alcohol dehydrogenase
1.1.1.245 cyclohexanol dehydrogenase


(R)-2,3-dihydroxy-3-methylbutanoate <=> 2-oxoisovalerate + H2O 4.2.1.9 dihydroxy-acid dehydratase

H+ + pyruvate <=> (S)-2-Hydroxy-2-methyl-3-oxobutanoate + CO2 2.2.1.6 acetolactate synthase
4.1.1.1 pyruvate decarboxylase


(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ <=> (S)-2-Hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ 1.1.1.383 ketol-acid reductoisomerase [NAD(P)+]
1.1.1.86 ketol-acid reductoisomerase (NADP+)


2-oxoisovalerate + H+ <=> Isobutyraldehyde + CO2 2.2.1.6 acetolactate synthase
4.1.1.1 pyruvate decarboxylase
4.1.1.7 benzoylformate decarboxylase
4.1.1.72 branched-chain-2-oxoacid decarboxylase




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